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Regulatory guidance for PK/PD analyses

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Regulatory guidance for PK/PD analyses

Status verified 2026-07-27. ICH guidelines are published openly and their requirements are summarised directly; always work from the authoritative copy for a submission.

ICH

GuidelineSubjectStatus
M12Drug interaction studiesStep 4 in 2024. FDA adopted 2 August 2024 with a Questions & Answers document; effective in the EU 30 November 2024; China 29 October 2024. First harmonised DDI guidance
M13ABioequivalence for immediate-release solid oral dosage formsStep 4 July 2024, effective 25 January 2025
M13BAdditional strengths and additional-strength biowaiversEndorsed 13 March 2025, Step 2b; consultation 9 April – 9 July 2025
M13CBE data analysis for highly variable drugs, narrow therapeutic index drugs, and complex designsBegins after M13B reaches Step 2. Reference-scaling remains regional until this lands
E11APediatric extrapolationStep 4 21 August 2024, effective 25 January 2025
M10Bioanalytical method validationThe assay behind every concentration; see the analytical-method-validation skill
E14Clinical evaluation of QT/QTc prolongationWith Q&A revisions; the 2022 Q&As added the double-negative nonclinical pathway
S7BNonclinical evaluation of QT prolongationPaired with E14 through the joint Q&As
E4Dose-response information to support registrationFoundational for exposure-response
E7Studies in support of special populations: geriatrics

FDA

GuidanceDateWhat it requires that gets missed
Population PharmacokineticsFinal, February 2022A prospective analysis plan; explicit BLQ handling; simulation-based diagnostics (VPC, pcVPC, NPC, NPDE). Notably states that model selection based on parameter shrinkage is not necessary
Optimizing the Dosage of Human Prescription Drugs and Biological Products for the Treatment of Oncologic Diseases (Project Optimus)Final, August 2024Identify a dosage maximising benefit-risk rather than the MTD; compare more than one dosage, randomised; a PK sampling and analysis plan in each protocol, sufficient for population PK and dose/exposure-response for safety and efficacy; early evaluation of intrinsic factors and DDIs
Exposure-Response Relationships2003Still the reference for E-R study design and analysis
Estimating the Maximum Safe Starting Dose in Initial Clinical Trials for Therapeutics in Adult Healthy Volunteers2005The body-surface-area HED conversion table (Km factors) used by allometry_and_fih.py
Physiologically Based Pharmacokinetic Analyses — Format and Content2018What a PBPK submission must contain
Clinical Pharmacology Considerations for Human Radiolabeled Mass Balance Studies
Renal and hepatic impairment guidancesStudy design, including the reduced/staged design

EMA

GuidelineSubject
Reporting the results of population pharmacokinetic analyses (EMA/CHMP/EWP/185990/2006)Structure and content of a popPK report
Investigation of bioequivalenceBeing superseded in scope by ICH M13A; EMA has published implementation considerations
Use of PBPK modelling and simulationQualification and reporting of PBPK
Reporting of physiologically based pharmacokinetic modelling and simulation
Evaluation of anticancer medicinal productsDose optimisation expectations parallel to Project Optimus

What each analysis type has to state

Non-compartmental analysis. Trapezoidal rule; BLQ rule at leading, embedded and trailing positions; the lambda_z selection rule with the window and point count per subject; whether AUCinf is observed- or predicted-based; exclusion criteria fixed before unblinding; software and version.

Population PK. A prospective analysis plan. Data assembly with exclusions and BLQ handling. Structural, statistical and covariate models with justification. Estimation method and software version. Diagnostics including a pcVPC. Parameter estimates with uncertainty. The model's intended use, and its qualification for that use. Deviations from the plan documented rather than absorbed.

Exposure-response. The exposure metric and why it is the mechanistically right one. Both efficacy and safety relationships. Explicit treatment of confounding between exposure and prognosis. The dose or exposure range covered by the data, and what is extrapolation.

Bioequivalence. Design and justification; log-transformed analysis; the 90% CI against pre-specified limits; the criterion (ABE, ABEL or RSABE) fixed in the protocol; the handling of dropouts and pre-dose concentrations; sample-size justification with its assumed GMR and CV.

PBPK. Platform and version; every drug parameter with its source and whether it was measured, predicted or optimised; the Kp prediction method; verification against observed clinical data before the untested application; sensitivity analysis on uncertain inputs; the model files.

DDI. The stepwise assessment with the basic-model results and cut-offs; what triggered further work; the mechanistic static or PBPK refinement with its verification; the clinical studies with index perpetrators and substrates; the labelling conclusion.

Model-informed drug development

Both FDA and EMA operate programmes for discussing model-based evidence before submission — FDA's MIDD Paired Meeting Program and EMA's Qualification of Novel Methodologies. Where a model is intended to replace a study rather than support one, engaging early is what determines whether the model is accepted. The level of rigour expected scales with what the model is being asked to carry.

The general principle

Regulators evaluate a model against its intended use, not in the abstract. A model adequate for choosing a Phase II dose is not automatically adequate for waiving a paediatric study or supporting a labelling claim. State the intended use first; the required evidence, verification and documentation follow from it.