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Ginkgo Cloud Lab

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Ginkgo Cloud Lab

Overview

Ginkgo Cloud Lab (https://cloud.ginkgo.bio) provides remote access to Ginkgo Bioworks' autonomous lab infrastructure. Protocols are executed on Reconfigurable Automation Carts (RACs) -- modular units with robotic arms, maglev sample transport, and industrial-grade software spanning 70+ instruments.

The platform also includes EstiMate, an AI agent that accepts human-language protocol descriptions and returns feasibility assessments and pricing for custom workflows beyond the listed protocols.

The catalog is organized into Expression & Purification (in vitro / cell-free / E. coli / Pichia), Characterization & Assay, Method & Target Onboarding, and Specialty. Pick a protocol below, then read its reference file for inputs, outputs, the automated workflow, and ordering details.

Available Protocols

Expression & Purification - In vitro

ProtocolReadoutPriceTurnaroundStatus
IVT mRNA/circRNA SynthesisqPCR (mRNA or circRNA, 384-well)$99/sampleup to 12 business daysCertified

Expression & Purification - Cell-free (E. coli CFPS)

ProtocolReadoutPriceTurnaroundStatus
Validate sequence expressionGo/no-go titer + purity (up to 1800 bp)$39/sampleup to 10 daysCertified
Optimize expression conditionsDoE across 24 conditions$199/sampleup to 11 daysCertified
Express + quantify (HiBiT)Luminescence, no purification$39/sampleup to 11 daysCertified
Express + purify (A280)Strep-tag, A280 yield$149/sampleup to 11 daysCertified
Express + purify minibinderStrep-tag, A280, LabChip$149/sampleup to 11 daysCertified
Express + purify (A280 + LabChip)Strep-tag, A280 + purity/size$159/sampleup to 12 daysCertified

Expression & Purification - E. coli

ProtocolReadoutPriceTurnaroundStatus
Express + quantify (HiBiT)Luminescence (up to 384 constructs)$79/sampleup to 3 weeksCertified
Express + purify (A280)His-tag, A280 yield$199/sampleup to 3 weeksCertified
Express + purify minibinderHis-tag, A280 yield$199/sampleup to 3 weeksCertified
Express + purify (A280 + LabChip)His-tag, A280 + purity/size$209/sampleup to 3 weeksCertified

Expression & Purification - Pichia

ProtocolReadoutPriceTurnaroundStatus
Express + quantify (LabChip)Secreted protein, size/purity (up to 96)$89/sampleup to 4 weeksCertified (New)

Characterization & Assay

ProtocolReadoutPriceTurnaroundStatus
Express + thermal shiftSYPRO Orange Tm (Tonset, TM1-3)$159/sampleup to 12 daysCertified
Detect enzymatic products (Echo-MS)Substrate/product by Echo-MS$44/sampleup to 13 daysBeta

Method & Target Onboarding

ProtocolReadoutPriceTurnaroundStatus
Onboard Echo-MS methodCalibration curve, LOD/LOQ$799/moleculeup to 3 weeksCertified
Onboard SPR targetValidated SPR capture method$1,399/targetup to 4 weeksBeta

Specialty

ProtocolReadoutPriceTurnaroundStatus
Generate fluorescent pixel artUV photo, 7-color E. coli palette$25/plateup to 7 daysBeta

Coming soon: Protein Expression and Binding Affinity Characterization (express + purify, then screen binding affinity against a target).

Choosing a Protocol

  • Quick expressibility screen? Cell-free HiBiT ($39) or Validate sequence expression ($39).
  • Need purified protein + yield? A280 tiers (cell-free or E. coli); add LabChip for purity/size.
  • Difficult / membrane / disulfide / cofactor targets? Cell-free Optimize (24-condition DoE).
  • Secreted or eukaryotic targets? Pichia expression.
  • Screening de novo binders/minibinders? Cell-free or E. coli minibinder tiers, then SPR onboarding for kinetics.
  • Enzyme activity / biocatalysis? Echo-MS enzymatic detection (onboard the analyte method first).
  • Stability / developability ranking? Thermal shift assay.
  • RNA (mRNA/circRNA)? IVT synthesis + qPCR.

General Ordering Workflow

  1. Select a protocol at https://cloud.ginkgo.bio/protocols
  2. Configure parameters (number of proteins/samples/molecules/targets, replicates, plates)
  3. Download the protocol's input template and upload inputs (FASTA/CSV/XLSX for sequence protocols; Design Tool for pixel art; vendor catalog numbers for onboarding)
  4. Add any special requirements in the Additional Details field
  5. Provide an email, agree to the protocol terms, and add to cart / submit to receive a feasibility report and price quote

For protocols not listed above, use the EstiMate chat (https://cloud.ginkgo.bio/estimate) to describe a custom protocol in plain language and receive a compatibility assessment and pricing.

Authentication

Access Ginkgo Cloud Lab at https://cloud.ginkgo.bio. Account creation or institutional access may be required. Contact Ginkgo at [email protected] for access questions.

Key Infrastructure

  • RACs (Reconfigurable Automation Carts): Modular robotic units with high-precision arms and maglev transport
  • Catalyst Software: Protocol orchestration, scheduling, parameterization, and real-time monitoring
  • 70+ integrated instruments: Agilent Bravo liquid handlers, Beckman/Labcyte Echo acoustic dispensers, BMG PHERAstar / Tecan Spark readers, Revvity LabChip, Bio-Rad CFX Opus, Nicoya Alto SPR, SciEx Echo-MS, Inheco/Cytomat incubators, and more
  • Nebula: Ginkgo's autonomous lab facility in Boston, MA