scientific-skills/ginkgo-cloud-lab/references/fluorescent-pixel-art-generation.md
URL: https://cloud.ginkgo.bio/protocols/fluorescent-pixel-art-generation Status: Beta Price: $25/plate Turnaround: 5-7 days
Transforms a digital image into a living, fluorescent bacterial artwork printed on an agar omni-tray. Customers submit a pixel art design and colors are mapped to distinct fluorescent E. coli strains. Overnight cultures are prepared from frozen glycerol stocks, diluted, and dispensed onto selective LB-chloramphenicol agar plates via Echo acoustic liquid handling at 50 nL per spot. Plates are incubated at 30 deg C for 16 hours, followed by 4 deg C for 12 hours to stabilize colony morphology and fluorescence. High-resolution photographs are captured under UV illumination and delivered digitally.
.png or .svg format| Strain/Protein | Color |
|---|---|
| sfGFP | Green |
| mRFP | Red |
| mKO2 | Orange |
| Venus | Yellow |
| Azurite | Blue |
| mClover3 | Bright Green |
| mJuniper | Dark Green |
| mTurquoise2 | Cyan |
| Electra2 | Electric Blue |
| mWasabi | Light Green |
| mScarlet-I | Scarlet |