Cell Free Protein Expression Validation

URL: https://cloud.ginkgo.bio/protocols/cell-free-protein-expression-validation Status: Ginkgo Certified Price: $39/sample (default: $936 for 8 proteins x 3 replicates = 24 samples) Turnaround: 5-10 days

Overview

Fastest path from a protein sequence to a quantitative go/no-go readout on expression. Uses a proprietary reconstituted E. coli transcription-translation (cell-free protein synthesis, CFPS) system. Reactions complete in 4-16 hours. Designed for early-stage screening, novel construct evaluation, and rapid triage of candidate sequences before committing resources to downstream optimization or purification.

Input

• DNA sequence in .fasta format
• Sequences up to 1800 bp supported

Output

• Expression Confirmation: Verification of target protein at expected molecular weight
• Baseline Titer: Initial quantitative yield measurement (mg/L)
• Initial Purity: Percentage of target protein vs. impurities, delivered with virtual gel images

Automated Workflow

Phase 1 - CFPS Reaction Setup & Incubation

1. Retrieve plates
2. Stamp DNA templates
3. Seal plate
4. Incubate shaking at 30 deg C

Phase 2 - Quantification Prep

1. Dispense PBS diluent
2. Seal plate
3. Store at 4 deg C

Phase 3 - LabChip Quantification

1. Unseal plate
2. LabChip quantification
3. Seal plate
4. Store at 4 deg C

Protocol Parameters

• Payloads & Reagents
• Bravo Stamp
• HiG Centrifuge
• Incubation & Storage

Ordering

• Number of Proteins: configurable
• Number of Replicates: configurable
• File Upload: CSV, Excel, FASTA, TXT, PDF, ZIP
• Additional Details: free-text field for special requirements

Certification Milestones

• Dry Run Complete
• Wet Run Complete
• Biovalidation Complete
• App Note Complete

Use Cases

• Screening candidate protein sequences for expressibility
• Go/no-go decisions before investing in optimization
• Evaluating novel constructs in a cell-free system
• Comparing expression levels across sequence variants