scientific-skills/deeptools/assets/quick_reference.md
bamCoverage --bam input.bam --outFileName output.bw \
--normalizeUsing RPGC --effectiveGenomeSize 2913022398 \
--binSize 10 --numberOfProcessors 8
bamCompare -b1 treatment.bam -b2 control.bam -o ratio.bw \
--operation log2 --scaleFactorsMethod readCount
multiBamSummary bins --bamfiles *.bam -o counts.npz
plotCorrelation -in counts.npz --corMethod pearson \
--whatToShow heatmap -o correlation.png
computeMatrix reference-point -S signal.bw -R genes.bed \
-b 3000 -a 3000 --referencePoint TSS -o matrix.gz
plotHeatmap -m matrix.gz -o heatmap.png
plotFingerprint -b input.bam chip.bam -o fingerprint.png \
--extendReads 200 --ignoreDuplicates
| Organism | Assembly | Size |
|---|---|---|
| Human | hg38 | 2913022398 |
| Mouse | mm10 | 2652783500 |
| Fly | dm6 | 142573017 |