scientific-skills/database-lookup/references/brenda.md
zeep library.https://www.brenda-enzymes.org/soap/brenda_zeep.wsdl
Free registration required at https://www.brenda-enzymes.org/register.php Credentials (email + SHA-256 hashed password) passed with every call.
All methods take email, password (SHA-256), and ecNumber as base parameters.
| Method | Description |
|---|---|
getKmValue | Michaelis constant (Km) |
getTurnoverNumber | Turnover number (kcat) |
getKcatKmValue | Catalytic efficiency (kcat/Km) |
getKiValue | Inhibition constant (Ki) |
getIc50Value | IC50 values |
getSpecificActivity | Specific activity |
getPhOptimum | pH optimum |
getTemperatureOptimum | Temperature optimum |
getSubstrate | Substrates |
getProduct | Products |
getInhibitors | Inhibitors |
getCofactor | Cofactors |
getOrganism | Source organisms |
getReaction | Reaction equations |
getSequence | Protein sequences |
getDisease | Associated diseases |
fieldName*value format. Empty value = return all.
ecNumber*1.1.1.1 # Required: EC number
organism*Homo sapiens # Optional: filter by organism
substrate*ethanol # Optional: filter by substrate
kmValue* # Return field (empty = all)
import hashlib
from zeep import Client
client = Client("https://www.brenda-enzymes.org/soap/brenda_zeep.wsdl")
email = "[email protected]"
password = hashlib.sha256("your_password".encode()).hexdigest()
# Get Km values for alcohol dehydrogenase
result = client.service.getKmValue(
email, password,
"ecNumber*1.1.1.1", "organism*Homo sapiens",
"kmValue*", "substrate*", "literature*"
)
Returns string parsed with ! (record separator) and #/* (field separators). Must be parsed manually.
No published limits. SOAP responses can take 1-5 seconds. Be respectful — free academic service.
Since BRENDA uses SOAP (not REST), making calls requires writing and executing a Python script with zeep. Use Bash to run the script rather than WebFetch.